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  • SupraSperm

    • SupraSpermTM

    Designed for safe and low risk sperm separation and purification by the density gradient method

    SupraSperm is an HEPES and Bicarbonate buffered medium containing silane-coated, colloid silica particles.

    It is designed to enables motile sperm to be separated from extraneous cells and seminal plasma and to select a subpopulation of cells with the highest capacity for fertilising an oocyte.

    Contains SSR® with insulin that is shown to be a pro-survival factor for sperm1, 2.

    Key Features

    • Suspension of colloidal silica particles coated with silane
    • With Human Serum Albumin: acts as an anti-sticking effect, antioxidant , scavenger of excess ions and reservoir for cholesterols, fatty acids and vitamins
    • For convenience available both as a ready-to-use gradient system (55% and 80%) and a 100% solution for individual preparation of gradients.
    • Contains SSR® with insulin

    This product is available for sale in Europe, the U.S.A, Canada and Australia as well as selected countries in the rest of the world.

    suprasperm-and-system-820

    Order Codes

    Reference Description Size Shelf Life Guarantee*
    10970060 SupraSperm™ 100 100% solution for custom gradient preparation 60 ml Minimum 52 weeks
    10972060 SupraSperm™ 100 100% solution for custom gradient preparation 2×60 ml Minimum 52 weeks
    10970500 SupraSperm™ 100 100% solution for custom gradient preparation 500 ml Minimum 52 weeks
    10922010 SupraSperm™ System 55% + 80% gradients 2×10 ml Minimum 8 weeks
    10922060 SupraSperm™ System 55% + 80% gradients 2×60 ml Minimum 8 weeks

    *Minimum time to expiry (from date of shipment from Denmark)

    Composition

    • Calcium Chloride
    • EDTA
    • Glucose
    • HEPES Free Acid
    • HEPES sodium salt
    • Human Albumin Solution
    • Milli RX Water
    • Magnesium Sulfate
    • Potassium Chloride
    • Potassium Phosphate, monobasic
    • Sodium Chloride
    • Sodium lactate
    • Sodium Pyruvate
    • 1092 only: Gentamicin sulphate
    • Phenol Red
    • Sodium phosphate
    • SSR® (Synthetic Serum Replacement)

    Quality Control Testing

    • Sterility tested (Ph. Eur., USP)
    • Osmolality tested (Ph. Eur., USP)
    • pH tested (Ph. Eur., USP)
    • Endotoxin tested ≤ 0.1 EU/ml (Ph. Eur., USP)
    • Sperm survival test

    References

    • 1. Curry, B. J. et al., (2014) Spermatozoa secrete insulin and it has recently been shown that insulin plays a role in capacitation of mammalian spermatozoa, enhancing motility and the acrosome reaction.” Androfert. P-104.
    • 2. Pujianto D.A. et al., (2010). Prolactin exerts a prosurvival effect on human spermatozoa via mechanisms that involve the stimulation of Akt phosphorylation and suppression of caspase activation and capacitation. Endocrinology. 151.