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  • SupraSperm
  • SupraSpermTM

    Designed for safe and low risk sperm separation and purification by the density gradient method

    SupraSperm is an HEPES and Bicarbonate buffered medium containing silane-coated, colloid silica particles.

    It is designed to enables motile sperm to be separated from extraneous cells and seminal plasma and to select a subpopulation of cells with the highest capacity for fertilising an oocyte.

    Contains SSR® with insulin that is shown to be a pro-survival factor for sperm1, 2.

    Key Features

    • Suspension of colloidal silica particles coated with silane
    • With Human Serum Albumin: acts as an anti-sticking effect, antioxidant , scavenger of excess ions and reservoir for cholesterols, fatty acids and vitamins
    • For convenience available both as a ready-to-use gradient system (55% and 80%) and a 100% solution for individual preparation of gradients.
    • Contains SSR® with insulin

    This product is available for sale in Europe, the U.S.A, Canada and Australia as well as selected countries in the rest of the world.


    Reference Description Size Shelf Life Guarantee*
    10970060 SupraSperm™ 100 100% solution for custom gradient preparation 60 ml Minimum 52 weeks
    10972060 SupraSperm™ 100 100% solution for custom gradient preparation 2×60 ml Minimum 52 weeks
    10970500 SupraSperm™ 100 100% solution for custom gradient preparation 500 ml Minimum 52 weeks
    10922010 SupraSperm™ System 55% + 80% gradients 2×10 ml Minimum 8 weeks
    10922060 SupraSperm™ System 55% + 80% gradients 2×60 ml Minimum 8 weeks

    *Minimum time to expiry (from date of shipment from Denmark)

    • Calcium Chloride
    • EDTA
    • Glucose
    • HEPES Free Acid
    • HEPES sodium salt
    • Human Albumin Solution
    • Milli RX Water
    • Magnesium Sulfate
    • Potassium Chloride
    • Potassium Phosphate, monobasic
    • Sodium Chloride
    • Sodium lactate
    • Sodium Pyruvate
    • 1092 only: Gentamicin sulphate
    • Phenol Red
    • Sodium phosphate
    • SSR® (Synthetic Serum Replacement)
    • Sterility tested (Ph. Eur., USP)
    • Osmolality tested (Ph. Eur., USP)
    • pH tested (Ph. Eur., USP)
    • Endotoxin tested ≤ 0.1 EU/ml (Ph. Eur., USP)
    • Sperm survival test
    • 1. Curry, B. J. et al., (2014) Spermatozoa secrete insulin and it has recently been shown that insulin plays a role in capacitation of mammalian spermatozoa, enhancing motility and the acrosome reaction.” Androfert. P-104.
    • 2. Pujianto D.A. et al., (2010). Prolactin exerts a prosurvival effect on human spermatozoa via mechanisms that involve the stimulation of Akt phosphorylation and suppression of caspase activation and capacitation. Endocrinology. 151.